Background, Aim and Scope:
Selenium (Se) is one of the most widely distributed elements of the earth's crust at low concentrations. The extensive use of Se-containing chemicals due to anthropogenic activities has increased the ecological risk of environmental compartments. In plants, unfavorable environmental conditions often lead to an increased formation of reactive oxygen species (ROS), and consequently plant antioxidant enzymatic systems have been proposed to be important in plant stress tolerance. The goal of this study was to find out the metabolic responses of plants to Se, to provide quantitative information whether exogenous Se has a beneficial role in plants, and to investigate the potential of vegetation management of Se for potential phytoremediation.
Materials and Methods:
Pre-rooted young plants of weeping willows (Salix babylonica L.) were grown hydroponically in growth chambers and treated with Na2SeO4 or Na2SeO3 at 24.0 ± 10C for 168 h. Five different treatment concentrations were used, ranging from 0.44 to 8.72 mg Se/L for the treatments exposed to SeO 3/4 and from 0.50 to 10.0 mg Se/L for the treatments exposed to SeO 3/3, respectively. Various physiological parameters of the plants were monitored to evaluate toxicity from exogenous Se exposure. At the end of the study, total Se in the hydroponic solution was analyzed by hydride generation-atomic fluorescence spectrometry (HG-AFS).
Results:
Both chemical forms of Se at low concentrations showed growth-promoting effects on plants. A significant decrease of transpiration rates and of soluble protein contents of plants was observed at higher Se concentrations after 168 h of exposure. Measurable change of superoxide dismutases (SOD) activity in leaves was only detected under high Se treatments. Catalase (CAT) activity was significantly affected by the Se application. Slight change of peroxidase (POD) activity was measured in all treatments, whereas significant inhibition of POD activity was detected for the plants exposed to SeO 3/3 of 10.0 mg Se/L. Se-induced stress appeared in all treatments, thus resulting in measurable increase of glutathione peroxidase (GSH-Px) activity of the plants. Although both chemical forms of Se were taken up by weeping willows efficiently, their uptake rates were different.
Discussion:
Of all measured parameters, POD and CAT activities in leaves were noted the most sensitive indicator for the plants exposed to SeO 3/4 and SeO 3/3, respectively. Deleterious effects on plant physiological functions due to Se application were not observed over 168 h of exposure. This is largely due to the fact that well-established antioxidant enzymatic systems in plants and higher activities of GSH-Px largely reduced the negative effects on plants; SeO 3/3 caused much more severe stress to plants than SeO 3/4 at higher Se application rates. The uptake mechanisms between the two chemical species were quite different.
Conclusions:
Neither visible toxic symptom nor metabolic lesion was observed at low concentrations of Se, probably due to the highly active and well established alternative enzymatic systems in weeping willows. All selected parameters for toxicity determination were correlated to Se application significantly, but metabolic responses of plants to SeO 3/4 and SeO 3/3 were quite different. GSH-Px in leaves was probably the principle enzyme responsible for stress reduction from Se exposure. Due to their different chemical properties, weeping willows showed a faster uptake rate for SeO 3/4 than for SeO 3/3.
Recommendations and
Perspectives:
Exogenous Se has a beneficial role in plants and vegetation management of Se is a potential remediation strategy in cleaning up Se-contaminated sites. Weeping willow has a sound potential for phytoremediation of Se-contaminated sediment and groundwater because the tree is not only tolerant to Se but also uptake the chemical species from the environment. Further investigation on the biochemical mechanism of Se metabolism will provide insight to the specific interactions between Se and plants on the molecular level. Weeping willow has a sound potential for phytoremediation of Se-contaminated sediment and groundwater because the tree is not only tolerant to Se but also uptakes chemical species from the environment.
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