DOI: http://dx.doi.org/10.1065/espr2002.05.118
We reported previously that trichodiene, a volatile
trichothecene derivative, was produced by a Stachybotrys isolate,
also known to produce highly cytotoxic, non-volatile, macrocyclic
trichothecenes (satrotoxins). We investigated the relationship
between the production of trichodiene and various
non-volatile trichothecenes for several molds.
Volatile metabolites were concentrated by adsorption on Tenax
TA and analyzed by GC/MS, while non-volatile metabolites were
separated by HPLC, derivatized and analyzed by GC/MS.
Stachybotrys chartarum isolates producing macrocyclic trichothecenes
secreted significantly larger amounts of trichodiene and
other sesquiterpenes than isolates which only produced simple
trichothecenes. The amounts of secreted trichodiene were relatively
small in all cases.
With the exception of Memnoniella, which excreted small
amounts of sesquiterpenes, the other isolates produced varying
amounts of sesquiterpenes, including trichodiene, as well as
simple tricothecenes, no detectable trichodiene, but large
amounts of griseofulvin derivatives.
In Stachybotrys there is apparently a correlation between trichodiene
and macrocyclic trichothecene production. In the remaining
isolates, there was no simple relationship between trichodiene
and non-volatile trichothecene synthesis.
Trichodiene is produced in larger amounts by Stachybotrys isolates,
which also produce satratoxins, but it will be difficult to
utilize this metabolite to detect toxic isolates in buildings due to
the relatively small amounts excreted. |
